This study establishes the toxicity identification evaluation (TIE) procedure for use in high-tech industry effluents. The procedure was effectively used to confirm metal ions as the toxicant in toxic real aqueous sample. Toxic and artificial aqueous samples prepared by adding organic pollutant (tetramethyl ammonium hydroxide, 4- chlorophenol) and metal pollutant (copper sulfate) were subjected to relevant physico-chemical treatment procedures first. The toxicants were correctly identified and confirmed thereafter. The results demonstrate the feasibility of using the established TIE procedure in high-tech industrial effluents. We used sodium dodecyl sulfate (SDS) as reference toxicant to evaluate the feasibility of using Japanese medaka fish for testing freshwater and seawater toxicity. The 96hr-LC50 average was 31.93 mg/L with 4.39% CV (n=5). Before testing, all medaka fish have to be domesticated in seawater with similar salinity to become 1.0 ± 0.2cm larva. The LC50 value decreases with increased salinity of seawater, indicating that in higher salinity the Medaka fish tolerance to SDS was reduced. However, similar 96hr-LC50 values were observed in both natural and artificial sea water spiked with SDS as reference toxicant. The results demonstrate the feasibility of using Japanese medaka fish for testing freshwater and seawater toxicity. We used SDS as reference toxicant to evaluate the feasibility of using Apocyclops royi invertebrate for testing seawater toxicity. The 48hr-LC50 average was 29.09 mg/L with 4.28% CV (n=5). The 48hr-LC50 difference due to SDS is small between the natural and artificial seawaters. The Apocyclops royi, possessing the advantages of easy culturing in laboratory and independent of seasonal effects, is feasible for testing seawater toxicity.

biological toxicity、toxicity identification evaluation、toxicity coefficient、organic pollutants、heavy metal、sea creatures